Serological diagnosis of Zika virus infections

Anti-Zika Virus ELISA/IIFT

Anti-Zika Virus ELISA (IgA or IgG or IgM) # EI 2668-9601 A or G or M

  • Highly specific test: virtually no cross-reactivity to other flaviviruses by use of a virus-specific antigen
  • Fully automatable

IIFT Arbovirus Fever Mosaic 2 (IgG or IgM) # FI 2668-1 G or M

  • Comprehensive syndrome and region-specific profile for differential diagnosis*
  • IIFT BIOCHIP mosaic includes infected cells with DENV: dengue virus (serotypes 1, 2, 3 and 4); CHIKV: chikungunya virus and ZIKV: Zika virus

Anti-Zika Virus IFA

*Cross-reactions to other flaviviruses cannot be excluded.

EUROIMMUN AG announces option agreement on Zika virus serology

EUROIMMUN and Roche announced that they have entered into an option agreement under which Roche gains access to intellectual property in the field of Zika virus immunodiagnostics assigned to EUROIMMUN.

Press Release


NEW: EUROIMMUN Anti-Zika Virus ELISA (IgA)

Anti-Zika virus IgA may indicate an acute infection in anti-Zika virus IgM-negative patients.

Especially in secondary flavivirus infections specific IgM - the classical marker of acute infection – is often found at low titer, sometimes being even undetectable, while synthesis of specific IgG is rapidly stimulated. Shortly after infection, the IgG titer then levels off at moderate concentration, indistinguishable from IgG titers in convalescent infections. Similar situations have also been observed in patients with Zika virus (ZIKV) infections, recently, making it difficult to discriminate between an acute secondary and a past infection. Since ZIKV is suspected to be associated with major neurological consequences (Guillan-Barré syndrome, microcephaly in newborns), diagnosis of acute infections though is highly crucial. We analysed the course of anti-ZIKV IgA, IgM and IgG titers in two confirmed patients for whom sequential serum samples were available asking whether IgA as alternative to IgM could indicate an acute phase of infection.

First data from the EUROIMMUN Anti-Zika virus ELISA (IgA) were presented at the IMED - International Meeting on Emerging Diseases and Surveillance (Vienna, Austria on November 4-7, 2016) and the 1st International Conference on Zika Virus in February 2017 in Washington DC, USA.


General Information

ELISA and indirect immunofluorescence tests (IIFT) have been developed for sensitive and specific detection of antibodies against Zika virus in patient serum samples. The assays are suitable for diagnosing acute infections as well as for disease surveillance. In particular, serological analysis can aid the differentiation of infections with Zika virus, dengue virus and chikungunya virus, which manifest with similar symptoms and are endemic in much the same geographic regions.
Serological tests provide a much longer window for diagnosis than direct detection methods. Detection of the virus by RT-PCR is only effective during the viraemic phase within the first week after onset of symptoms, and may already be negative by the time a patient consults their doctor. Anti-Zika virus antibodies appear around day 4 to 7 after symptom onset. IgM antibodies reach their peak after two to three weeks and remain detectable for several months, while IgG are assumed to persist life-long. The detection of specific IgM antibodies or a significant rise in the specific IgG titer in a pair of samples taken at least two weeks apart is evidence of an acute infection.

EUROIMMUN test systems for the diagnosis of Zika virus infections


ELISA

Anti-Zika Virus ELISA (IgA or IgM or IgG) provide fully automated antibody detection using microplates coated with a recombinant protein from Zika virus. This highly specific antigen avoids cross-reactivity with other flaviviruses. Preliminary data from panels of serologically well characterised sera indicate that there is no cross-reactivity with flaviruses like dengue, West Nile and Japanese encephalitis viruses. Larger studies are underway to confirm this.

Anti-Zika Virus ELISA (IgA)

Anti-Zika Virus ELISA (IgM)

Anti-Zika Virus ELISA (IgG)


IIFT

Anti-Zika Virus IIFT (IgM or IgG) utilise Zika virus-infected cells as the antigenic substrate. Positive and negative results are evaluated by fluorescence microscopy. With the Arbovirus Fever Mosaic 2 the Zika virus substrate is incubated in parallel with substrates for chikungunya virus and dengue virus serotypes 1 to 4. This BIOCHIP combination can help in differential diagnostic delimitation of Zika, dengue and chikungunya virus infections. Due to the use of whole virus particles, cross reactivities between flavivirus antibodies such as dengue and Zika virus can occur.

Serological differential diagnosis using IFA technology



Ordering products

For order information please contact your local distributor

Find distributor


Dr. Konstanze Stiba
+49 451 5855 25461
k.stiba@euroimmun.de

Product Manager Emerging
Infections


Publications

No molecular or serological evidence of Zikavirus infection among healthy blood donors living in or travelling to regions where Aedes albopictus circulates.
Borena W, Hofer T, Stiasny K, Aberle SW, Gaber M, von Laer D, Schennach H. PLoS ONE 2017 12(5): e0178175.
Complete laboratory diagnosis of Zika virus infections
J. Gosink. CLINICAL LABORATORY INTERNATIONAL. June 2017.
Serodiagnosis of Zika virus infections by a novel NS1-based ELISA devoid of cross-reactivity with dengue virus antibodies. A multicohort study of assay performance, 2015 to 2016.
Steinhagen K, Probst C, Radzimski C, Schmidt-Chanasit J, Emmerich P, van Esbroeck M, Schinkel J, Grobusch MP, Goorhuis A, Warnecke JM, Lattwein E, Komorowski L, Deerberg A, Saschenbrecker S, Stöcker W, Schlumberger W. 2016;21(50):pii=30426.
High specificity of a novel Zika virus ELISA in European patients after exposure to different flaviviruses
Huzly D, Hanselmann I, Schmidt-Chanasit J, Panning M. High specificity of a novel Zika virus ELISA in European patients after exposure to different flaviviruses. Euro Surveill. 2016;21(16):pii=30203.
Zika virus Antibody Detection: Evaluation of Three Different Serologic Methodologies
Granger D, Gomez LJ, Schimek M, Dubbels M, Mosquera JA, Christensen J, Bistodeau S, Strain A, Theel ES. Zika virus Antibody Detection: Evaluation of Three Different Serologic Methodologies. Poster at CVS 2016 (USA)
Sexual transmission of Zika virus in Germany, April 2016
Reusken C, Pas S, GeurtsvanKessel C, Mögling R, van Kampen J, Langerak T, Koopmans M, van der Eijk A, van Gorp E. Longitudinal follow-up of Zika virus RNA in semen of a traveller returning from Barbados to the Netherlands with Zika virus disease, March 2016. Euro Surveill. 2016;21(23):pii=30251.
Longitudinal follow-up of Zika virus RNA in semen of a traveller returning from Barbados to the Netherlands with Zika virus disease, March 2016.
Reusken C, Pas S, GeurtsvanKessel C, Mögling R, van Kampen J, Langerak T, Koopmans M, van der Eijk A, van Gorp E. Longitudinal follow-up of Zika virus RNA in semen of a traveller returning from Barbados to the Netherlands with Zika virus disease, March 2016. Euro Surveill. 2016;21(23):pii=30251.
Zika virus infection in two travelers returning from an epidemic area to Italy, 2016: Algorithm for diagnosis and recommendations
Calleri G, Burdino E, Bonora S, Raso R. Zika virus infection in two travelers returning from an epidemic area to Italy, 2016: Algorithm for diagnosis and recommendations, Travel Medicine and Infectious Disease (2016).
Zika Virus Disease in Traveler Returning from Vietnam to Israel
Meltzer E, Lustig Y, Leshem E, Levy R, Gottesman G, Weissmann R, Rabi DH, Hindiyeh M, Koren R, Mendelson E, Schwartz E. Zika Virus Disease in Traveler Returning from Vietnam to Israel, Emerging Infectious Diseases 2016; 22 (8).
Miscarriage associated with Zika virus infection
van der Eijk AA, van Genderen PJ, Verdijk RM, et al. Miscarriage associated with Zika virus infection. N Engl J Med 2016;375:1002-4.
Infection dynamics in a traveller with persistent shedding of Zika virus RNA in semen for six months after returning from Haiti to Italy
Barzon L, Pacenti M, Franchin E, Lavezzo E, Trevisan M, Sgarabotto D, Palù G. Infection dynamics in a traveller with persistent shedding of Zika virus RNA in semen for six months after returning from Haiti to Italy, January 2016. Euro Surveill. 2016;21(32):pii=30316

The German Society of Gynaecology and Obstetrics (DGGG) recommends test systems based on Zika virus NS1 antigen for the diagnosis of Zika virus infections.
231. DGGG Statement, p. 4, 2nd para. (only available in German): "Due to the antigenic similarity with other Flaviviruses (e.g. dengue virus), significant cross reactions can occur in the IIFT (based on whole virus antigen). This effect is considerably less pronounced in the NS1 EIA* (a recombinant protein is used as antigen) owing to the high specificity (Huzly et al., 2016).
*EUROIMMUN Anti-Zika-Virus ELISA

The Growing Global Threat of Zika, Dengue and Chikungunya Viruses
Medlab Magazine. 2016

Isolation of infectious Zika virus from saliva and prolonged viral RNA shedding in a traveller returning from the Dominican Republic to Italy, January 2016
Euro Surveill. 2016;21(10)